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nine eliquid studies

Top 9 Studies on E-Liquid You Need to Know About

It goes without saying that there is a lot of controversy about e-cigarettes and e-juice. With new scientific evidence emerging regularly, we’re finding out more and more each month, but there is an inherent tendency for benefits to be swept aside in favor of exaggerated portrayals of potential risk in the media.

If you’re finding yourself unsure what to think about the state of the science on e-liquid, learning more about some of the most important studies conducted to date gives you an objective picture of what we know and what we still need to find out.

Here are 9 studies addressing e-liquid (or closely-related issues) that you need to know about.

9 – Peering Through the Mist – the Chemistry of E-Cigarettes

This paper, written by Igor Burstyn and funded by CASAA, provides a systematic review of the evidence on the chemistry of the aerosol (i.e. the “vapor”) produced from e-liquids. The research compared the levels of chemicals to “threshold limit values” (TLVs), which are internationally-recognized workplace exposure limits designed to minimize risks to employees. The findings showed that almost all chemicals given off by vaporized e-liquids resulted in an exposure much lower than 1 percent of the TLV, with acrolein and formaldehyde exposure being less than 5 percent of the TLV, suggesting that e-liquids are very low in risk from a chemical perspective. The author found that only exposure to the main ingredients, PG and VG, may warrant concern, primarily due to the lack of established toxicity for these chemicals (although they’re generally recognized as safe).

8 – How Addictive Are E-Liquids?

Researchers on this study aimed to determine how addictive e-cigarettes are in comparison to tobacco cigarettes and nicotine gum. The study showed that those using nicotine-containing liquids were slightly more addicted than those using no-nicotine e-liquids, and that – among former smokers – long-term vapers (defined as use for over three months) were less addicted to e-cigarettes than long-term nicotine gum users were to the gum. For short-term users, there was little difference between gum users and vapers. Dual users (who both vaped and smoked) were generally less addicted to e-cigs than smokers who didn’t vape were to cigarettes.

7 – Do E-Liquids Poison More People Than Cigarettes?

This study used data from U.S. poison control centers to determine whether e-liquid poisonings were a concern, particularly in comparison to cigarette poisonings. Over the studied time period, exposures to e-cigs and e-liquids increased from one per month in September 2010 to 215 per month in February 2014, but cigarette-related exposures were consistently higher, ranging from 301 to 512 calls per month. For cigarettes, the vast majority of potential poisonings (since not all calls represent serious poisonings) were in 0 to 5 year olds (around 95 percent of calls), but this age group only accounted for 51 percent of calls related to e-cigarettes. Overall, e-cigarette poisonings increased, but are still dwarfed by cigarette-related calls and those from other household substances.

6 – Do E-Liquid Flavors Attract Non-Smoking Teens to Vaping?

This study set out to address the widespread concern that e-cig liquid flavors such as bubblegum will attract teens who otherwise wouldn’t smoke to vaping. The researchers compared the level of interest in trying e-cigarettes with a specific flavor of liquid to the level of interest in trying the same flavor when attached to bottled water or ice cream, comparing non-smoking teens with smoking adults and ranking interest in trying on a 0 to 10 scale. For non-smoking teens, the average level of interest in e-cigarettes was 0.41 (very low), and this didn’t significantly vary according to flavor. Adult smokers’ interest was higher and did significantly vary by flavor. This study suggests that e-liquid flavors don’t lure otherwise non-nicotine-consuming youth into vaping, despite concerns, but may pique the interest of adult smokers.

5 – Should Vapers Be Worried About Inhalation of Flavor Chemicals?

This study involved looking at the levels of flavoring chemicals in a sample of 30 e-liquids. The results showed that the majority of tested e-liquids contained less than 1 percent flavorings by weight, with 2 e-liquids containing more than 3 percent flavorings. Of the 24 identified chemicals, 6 of them were aldehydes, known to be primary irritants of the respiratory tract (although this doesn’t necessarily indicate a health risk). The researchers also noted that the majority of the tobacco-flavored e-liquids studied used confectionary flavor chemicals rather than tobacco extracts.

The authors performed some calculations, indicating that the e-liquids with the highest amounts of benzaldehyde and vanillin in the whole study would expose users to more than the recommended workplace exposure for those chemicals, although the average levels found in the study suggest that vapers’ exposure is lower than the workplace limits.

4 – Is the Nicotine Labeling on E-Liquids Accurate?

The researchers on this study gathered a sample of e-liquids from around the world – 32 from the U.S., 29 from South Korea and 30 from Poland – and analyzed the nicotine levels contained within them. The results showed that, despite much concern about unreliable nicotine levels in e-liquids, the vast majority of samples didn’t vary significantly from the amount indicated on their label. In the whole sample, 19 percent of all e-liquids had discrepancies of over 20 percent between their labeled and actual nicotine content. In the U.S. samples, the level of nicotine detected varied between 0 and 3.66 percent, but the researchers found that three products labeled as nicotine free did contain detectable traces of nicotine. Overall, it shows that while most e-liquids are labeled accurately, mixers should take extra care to ensure accurate nicotine content.

3 – Is E-Liquid Toxic to Cells?

This toxicological study looked at 20 different e-liquid samples, and set out to determine if the vapor produced from them was toxic to heart cells, and offered a comparison with cigarette smoke. The researchers found that cigarette smoke was toxic to cells at concentrations higher than 6.25 percent, but almost all e-liquids weren’t toxic at any concentration. However, four e-liquids were toxic to cells at the highest concentrations, and this suggests that the toxicity of e-liquid varies according to flavor, rather than nicotine concentration or the base ingredients (PG or VG) used (this was confirmed using a sample not containing flavors or nicotine). Overall, the authors conclude that all e-liquids are significantly less toxic to heart cells than cigarette smoke.

2 – Do Flavors Matter for Vapers?

With all the concern about flavors attracting children to vaping (despite the study already mentioned that found this not to be true), this study aimed to determine whether removing available flavors would impact on ordinary adult consumers. Over 4,600 vapers answered an online survey, looking at their smoking status and their views on flavored e-liquids, with particular consideration to their role in helping them quit smoking (as over 90 percent of the sample had).

The results showed that successful quitters switched flavors more than those still smoking, with almost 70 percent of them doing so daily or more. The respondents classed the variety of flavors available as “very important” for quitting smoking, and most said that restrictions on available flavors would make vaping less enjoyable. In addition, almost half said reducing the flavors on offer would increase their cravings for cigarettes, and just under 40 percent said they would have been less able to quit or reduce smoking without them. In short, the study showed that reducing the availability of flavors could drastically impact the potential of e-cigarettes to help smokers quit.

1 – How Many E-Liquids Contain Diacetyl?

One of the core issues for vaping is the potential for risks related to specific flavors, and diacetyl (a buttery flavor) is the poster-chemical for this danger, being associated with a rare condition impacting on lung functioning. This study took a sample of 159 e-liquids (from 36 manufacturers) and tested them for diacetyl and acetyl propionyl (a similar chemical that likely has similar risks), choosing liquids that potentially contained the chemical due to their flavor descriptions. The results showed that a huge 74.2 percent of samples contained either diacetyl or acetyl propionyl, sometimes despite assurances to the contrary. The median daily exposure for a vaper was calculated as being slightly lower than the recommended maximum workplace exposure, but almost half of diacetyl-containing and over two-fifths of acetyl propionyl-containing samples exceeded these limits. Calculated levels of diacetyl exposure from vaping were 100 times lower than smoking, and for acetyl propionyl it was 10 times lower than from smoking. Regardless, the authors identified these chemicals as a completely avoidable risk for vapers.

Conclusion – We Know a Lot, But We Need to Know More

Potentially the most important thing we need more information on is the risk associated with different flavoring chemicals, because the more we find out, the more responsible mixers can avoid sources of unnecessary risk. With diacetyl in particular, companies that openly publish results of lab testing of their e-liquids should be favored by vapers, because assurances of “no diacetyl” aren’t always reliable.

The available research might show some areas where we need more data, but it does go a long way to addressing some myths about vaping. It’s clear that claims of e-liquid flavors attracting non-smoking youths to vaping are not backed by available evidence, nor are concerns about e-liquid poisonings, as well as claims that when you buy e-liquid is just as addictive as cigarettes. If you’ve learned nothing else, it should be that it’s better to base your opinions on the science than over-hyped media reporting of the findings.

Michael Grey
Find Me Here

Michael Grey

With a passion to educate through my writings and a passion to help others, I found my place here at Black Note. Being that I am a former smoker who quit smoking because of vaping, I knew I could help others do the same. Working for Black Note is a true blessing. Not only do I help educate vapers on a daily basis through my writings, but I am also enjoying the benefits of working with an amazing team and sharing an authentic tobacco solution with the vaping community.
Michael Grey
Find Me Here

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What is Naturally Extracted Tobacco E-Liquid?

E-juice, also known as e-liquid or vaping liquid, is the liquid that’s placed in the vaping device and subsequently atomized into vapor. When you go to buy e-juice, you’ll notice it contains a mix of several ingredients. These typically include vegetable glycerin (VG), propylene glycol (PG), some type of flavoring, and nicotine.

VG and PG form the base of the e-liquid. VG is a component used in food and personal care products; its role in e-liquid is to create a thick, dense vapor. PG is another popular solvent used in a variety of everyday items. It carries the e-liquid flavor, provides the “throat hit,” and enhances the vaping liquid’s ability to soak into wicks or cartomizer materials.

Nicotine is an optional ingredient, often available in different levels. E-juice also contains some type of flavoring.

Black Note leads the pack of tobacco vaping liquid by using only natural flavoring – extract from real tobacco leaves to produce real tobacco taste. When you go to buy e-juice from other companies, you may find any number of chemicals used to create synthetic flavors that include fake strawberry, manufactured vanilla, and even man-made tobacco flavors.

If your aim when you buy e-liquid is to experience the subtle nuances, genuine flair and authentic taste of real tobacco, then you’ll be on the mark if you buy e-juice from the Black Note lineup.

Best E-Juice

When you’re going to buy e-liquid, it’s only natural you’ll want the best e-liquid. But what, exactly, does it “best e-juice” mean? We’ll tell you.

For starters, you’ll want an e-juice that doesn’t contain all kinds of strange chemicals and concoctions that you can’t even pronounce. True, even the best e-liquid will contain vegetable glycerin (VG) and proplene glycol (PG), which serve as the base of e-juice, but you don’t necessarily need a host of other chemical compounds.

The best e-juice will stick to natural ingredients, like real tobacco extract, rather than rely on synthetic flavorings, colorings and other additives. Diacetyl is one of those synthetic ingredients that has gotten a pretty bad rap, although its use is not all that uncommon for producing sweet e juice flavors.

Definitely read the ingredient list before you buy e-juice to ensure you recognize what’s on it. Better yet, see if the e-juice company offers a lab report to confirm what’s actually inside the e-liquid.

In addition to having as few chemical ingredients as possible, the best e-juice will have an amazing taste. For those who enjoy authenticity over imitation, the most amazing taste is the most natural taste, especially when it comes to tobacco. Since Black Note uses real extract from tobacco leaves, we not only avoid all those weird chemical flavorings, but we also produce the most authentic experience: tobacco vaping liquid that actually tastes like tobacco.

Best E-Liquid on the Market

Anyone looking for the best e-liquid on the market need look no further than Black Note. Black Note tobacco vaping liquid ranks as the best e-juice across the board in every category, starting with the taste.

Black Note’s main claim to fame is producing a tobacco vaping liquid that actually tastes like real tobacco. That because we use real tobacco extract, not chemicals, to create the best e-juice tobacco flavor. Using extract from real tobacco leaves retains tobacco’s nuances and flavor notes, giving you the most authentic and multi-faceted tobacco experience.

In addition to being the best e-juice for tobacco taste, Black Note also has the best e-liquid manufacturing process. We spent years researching and testing various production methods, and finally landed on one that we believe is absolutely perfect.

It starts with growing carefully selected tobacco seeds, followed by an equally precise of steps that include an extensive natural extraction process. Even our bottling and packaging is done with the utmost care, using recycled and recyclable packaging materials to ensure our products are as earth-friendly as they are vapor-friendly.

One more category where Black Note ranks as the best e-liquid is with the value. Every order comes with free domestic shipping, one-to-three day domestic shipping guarantee, and a 90-day, money-back satisfaction guarantee. If you’re not happy for any reason, or don’t agree we’re the best e-juice on the market, send it back on us and we’ll refund your money.

Vape Juice Ingredients

Vape juice ingredients can be pretty straightforward – or not. At the very least, most vape juice contains propylene glycol (PG), vegetable glycerin (VG), and some type of flavoring. Nicotine has largely become an optional ingredient that can be included at varying levels or left out altogether.

Vegetable glycerin (VG) and propylene glycol (PG) are usually the two main ingredients that make up the base of most vape juice.

VG is a plant-based substance with the ability to produce a dense, thick vapor. PG is an additive found in many food items and certain medicines. It carries the flavoring, provides the “throat hit” and enhances the vape juice’s ability to soak into the wicking materials.

Nicotine levels can vary, as can ingredients that make up the flavorings. The components that make up vape juice flavoring are where ingredients can get rather complicated.

Vape juice that relies on artificial flavorings can contain any number of extraneous chemicals and other additives, whatever it takes to recreate the flavor the ecig juice is going for.

Black Note uses only natural flavorings: extract from real tobacco leaves. That keeps our ingredient list incredibly simple, backed by a lab report to prove it. You won’t find any strange chemical concoctions used to produce our tobacco flavor, just real tobacco extract that delivers a real tobacco experience.

What is the Best Flavor for Ecig Juice?

From strawberry shortcake to banana rum, ecig juice comes in tons of flavors. While folks can debate and discuss all the fruity, sweet or food-like flavors all day long, the best flavor for e-cig liquid is straight-up tobacco.

Why? Vaping was originally created as an alternative for traditional cigarettes, mainly for those who enjoy the taste of tobacco. And when you can find an e-cig liquid that is able to recreate tobacco’s full-bodied yet delicate flavor notes and nuances, but without the harmful chemicals found in cigarettes, then you’ve found the best ecig juice.

Recreating tobacco’s complex flavor isn’t easy, especially when it’s attempted with artificial ingredients. That’s why Black Note sticks to the real thing, extracting the essence of real tobacco leaves to flavor our vaping liquid. Not only does this avoid the harmful chemicals in cigarettes, but it also eliminates the flavoring chemicals found in many ecig juices. The end result is real tobacco taste, an authentic tobacco experience and the best flavor for ecig juice you can find.

Notice also we said the best e-cig flavor was straight-up tobacco, not plain old tobacco. Black Note’s real tobacco vaping liquid happens to come in a variety of different blends, from the light and airy to the peppery and robust. Not all tobacco is created equal, and different blends capture the specialized subtleties that make each tobacco unique.

Best Sweet Tobacco E-Juice

When it comes to sweet tobacco e-juice, your options can be broken down into two categories.

The first category contains tobacco e-juice that overlays the tobacco flavor with artificial chocolate, caramel, vanilla, fruity or other dessert-like flavorings. We wouldn’t classify any such flavors as the best tobacco e liquid, simply because they typically use chemicals and other artificial ingredients to create the tobacco taste, the sweet taste, or both.

The second category of sweet tobacco e-juice is where you’ll find the best tobacco e-liquid, sweet or otherwise. This category contains vaping liquid created using only natural flavorings, which is exactly where Black Note fits in. Instead of concocting flavors using synthetic ingredients, Black Note uses an extensive extraction process that slowly and deliberately extracts the tobacco essence from real tobacco leaves.

The result is the best tobacco e-juice: tobacco vaping liquid that actually tastes like real tobacco. And if you’re going for the best sweet tobacco e liquid, we have a few tobacco blends that offer varying levels of sweetness.

Prelude is our sweetest tobacco e-liquid, containing golden Virginia tobacco. Made from fire-cured dark Virginia tobacco, Sonata is our Cavendish blend with a mildly sweet and nutty flavor. Solo is our menthol blend, giving you a semi-sweet minty taste created from natural menthol crystals extract from dried mint leaves.

For the best tobacco e-liquid that’s naturally sweet and remarkably delicious, Black Note is at your service.

WARNING: Black Note products are not smoking cessation products and have not been tested as such. Black Note products are intended for use by adults of legal smoking age (21 or older in California), and not by children or women who are pregnant or breastfeeding. Black Note products contain nicotine, a chemical known to the State of California to cause birth defects or other reproductive harm. Ingestion of the non-vaporized concentrated ingredients can be poisonous. Keep out of reach of children and pets.

WARNING: This product contains nicotine. Nicotine is an addictive chemical.